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KMID : 0381120140360050599
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Genes and Genomics
2014 Volume.36 No. 5 p.599 ~ p.609
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Myostatin-2 isolation and spatiotemporal expression comparison between myostatin-1 and -2 in Larimichthys crocea
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Liu Zhifei
Xue Liangyi
Sun Sheng
Xu Zhen
Yu Hong
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Abstract
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The 3,110-bp myostatin-2 was obtained from a marine teleost, Larimichthys crocea (L. crocea), by PCR amplication coupled with 5′- and 3′-rapid amplification of cDNA ends. Myostatin-2 contains a 71-bp 5′-untranslated region, a 328-bp exon I, an 895-bp intron I, a 371-bp exon II, a 382-bp intron II, a 381-bp exon III, and a 682 bp 3′-untranslated region, and it encodes a protein of 359 amino acid residues. The deduced amino acid sequence has typical characteristics of TGF-β family members with nine conserved cysteine residues and a RXXR proteolytic processing site within the conserved C-terminal portion, and more than 90 % similarity with other fish myostatin-2 proteins. Quantitative assessment revealed that levels of myostatin-1 transcripts fluctuated markedly, whereas myostatin-2 expression remained very low during most stages of embryonic development. In addition, both myostatin-1 and -2 transcripts were detected in all 10 examined tissues of juvenile and adult individuals, but they exhibited different expression patterns and responses to fasting. Expression of myostatin-1 was highest in skeletal muscle, which was higher than myostatin-2 by 2?3 orders of magnitude. Myostatin-2 transcripts were greatest in brain. Myostatin-1 expression increased significantly after 3 days of fasting, whereas myostatin-2 expression was unaffected. Our results imply that myostatin-1 and -2 may have different roles in skeletal muscle growth and development of L. crocea.
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KEYWORD
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Myostatin, Gene expression, Fasting, Larimichthys crocea
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